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    • “最新研究揭示自噬在电离辐射诱导神经胶质瘤细胞纤毛发生中的关键作用，为神经胶质瘤治疗提供新思路。”

      JIN Liangliang,YU Feifei,ZHANG Tongshan,HE Jinpeng,YANG Yanli

      Corrected Proof
      DOI：10.11889/j.1000-3436.2023-0104
      摘要：The role of autophagy in ionizing radiation (IR)-induced primary ciliogenesis in glioblastoma cells was explored. M059K and M059J cells underwent treatment with either 10 Gy of X-rays or serum starvation (SS) for three days. Subsequently, the primary cilia were examined through immunofluorescence staining targeting ciliary markers Arl13b and γ-tubulin. Cellular autophagy levels were estimated by immunofluorescence staining for the autophagy marker LC3 and immunoblotting analysis of p62. To assess primary ciliogenesis, M059K and M059J cells were treated with chloroquine (CQ) or rapamycin (RAPA), which respectively suppressed or activated cellular autophagy. The findings revealed that in M059K, the proportion of ciliated cells was approximately 40%, and this proportion increased to over 75% on day 3 post-irradiation in cells treated with 10 Gy of X-rays . However, the frequency of primary cilia in M059J cells (~7%) showed no obvious changes before and after X-ray irradiation. Interestingly, the proportion of ciliated cells boosted to approximately 80%and 50% in M059K and M059J cells starved for 3 d, respectively. Serum starvation (SS) elevated cellular autophagy levels in both cell lines, whereas X-ray irradiation enhanced autophagy levels only in M059K but not in M059J cells. Moreover, activation of autophagy using RAPA facilitated ciliogenesis in both cell lines, while the suppression of autophagy with CQ mitigated IR-induced cilia formation in M059K and SS-induced cilia formation in M059J cells. In summary, these results demonstrate that different cellular autophagy responses to IR in M059K and M059J cells participated in primary ciliogenesis elicited by IR exposure.  
      关键词：Autophagy;Ionizing radiation;Primary cilia;Glioblastoma   
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      更新时间：2024-07-11

    • Xiao Hu,Jun Guo,Jun Wang,Jie Ma,Luming Zhao,Seongwoo Yoo,Dingyuan Tang

      Corrected Proof
      DOI：10.1038/s41377-023-01074-w
        
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      更新时间：2024-07-11
    • “海南大学儋州校区农科专业利用无人机进行大范围植被调查，提升教学实践效率与效果，对植被保护和探索无人机应用场景具有重大意义。”

      Li Dehan,Bi Chenchen,Jiao Ze′en,Lu Shijun

      Corrected Proof
      摘要：In recent years, the rapid development of Unmanned Aerial Vehicle (UAV) technology, coupled with its advantages of simple operation, makes it widely used in many fields. This paper discusses the application of UAV in the teaching practice of agriculture in Danzhou campus of Hainan university. The paper uses UAV to carry out large-scale vegetation survey, which is of great significance not only for improving teaching practice efficiency and effect, but also for vegetation protection and even for exploring more application scenarios of UAV.  
      关键词：Unmanned Aerial Vehicle   
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      更新时间：2024-07-11
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